Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTASQQQIQLARRPQGIPVHEDFRFET----IPVPEPKQGEVLVKTLYVSVDPYMRGRM----QDTKSYVEPFALDKALSGGVIAEVVSDGN-HLKKGDIVIGNLSWQEFSAVS--ESALRKIDTSLAPASAYLGILGMTGLTAYFGLLDIGRPKEGETVVVSGAAGAVGSTVGQIAKIKGARVVGIAGSDEKIDYLKQELQFDEAINYKTADDIQKALQNACPDGVDVYFDNVGGPISDAVMNLLNEFARIPVCGAISSYNAESEADDMGPRVQSKLIKTKSLMQGFIVSDYSDRFSEGAKQLAEWLKAGKLHYEETITEGFENIPDAFLGLFKGENKGKQLIKVSDPS 2J3J Chain:B ((1-343)) MTATNKQVILKDYVSGFPTESDFDFTTTTVELRVPE-GTNSVLVKNLYLSCDPYMRIRMGKPDPSTAALAQAYTPGQPIQGYGVSRIIESGHPDYKKGDLLWGIVAWEEYSVITPMTHAHFKIQHTDVPLSYYTGLLGMPGMTAYAGFYEVCSPKEGETVYVSAASGAVGQLVGQLAKMMGCYVVGSAGSKEKVDLLKTKFGFDDAFNYKEESDLTAALKRCFPNGIDIYFENVGGKMLDAVLVNMNMHGRIAVCGMISQYNLENQE---GVHNLSNIIYKRNRIQGFVVSDFYDKYSKFLEFVLPHIREGKITYVEDVADGLEKAPEALVGLFHGKNVGKQVVVVA---

General information: TITO was launched using: RESULT: Template: 2J3J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1890 -186892 -98.88 -562.93 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : -98.88 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.505

(partial model without unconserved sides chains): PDB file : Tito_2J3J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2J3J-query.scw PDB file : Tito_Scwrl_2J3J.pdb: