Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence METFKSILHEAQRIVVLTGAGMSTESGIPDFRSAGGIWTE------DASRMEAM-SLDYFLSYPRLFWPKFKELFQMKMSGSFEPNEGHLLLAELEKQGKQVDIFTQNIDGLHKKAGSRHVYELHGSIQTAACPACGARYDLP----HLLEREVPECTAAGNNGDICGTVLKTDVVLFGDAV---MHFDTLYEKLDQADLLLVIGTSLEVAPARFVPEDASLIPGMKKVIINLEPTY-----------CDSLFDMVIHQKIGEFARSLGMKK 4IF6 Chain:A ((14-270)) IEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDFPDLPDPQAMFDIEYFRKDPRPFFKFAKEIY----PGQFQPSLCHKFIALSDKEGKLLRNYTQNIDTLEQVAGIQRIIQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPL--AIMKPEIVFFGENLPEQFHRAMKYDK-DEVDLLIVIGSSLKVRPVALIP--SSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAK------

General information: TITO was launched using: RESULT: Template: 4IF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1152 -135468 -117.59 -583.91 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -117.59 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.536

(partial model without unconserved sides chains): PDB file : Tito_4IF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4IF6-query.scw PDB file : Tito_Scwrl_4IF6.pdb: