Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGIAGTFIFMIVIGAAIGAVTNHLAIQMLFRPYKAYYLFGKRVPFTPGLIPRRRDELA----KQMGLMVVNHLLTPEGIKKRLVS-DAAKTQALRVGEQLIQKLSLSEVTVKEALEKAGMKRPEKAADAWISSW-TDDKLHELFRQYGDQSL--KELVPIEVQEKLEEKIPMISGYILSRSVRYFESDEGKIRLGNMIDDFLKERGMLGSMVQLFLGNSSLADR-VLPELLKFLRNEETNKLLSDLLKNEWGKLR-EYTFNEADEKWNAKA---LIFSLK-RRVLQAFSTAPFFNNTIGTLTVRYESELTQQMLPALLDKLLEGISSNLESVLKRLRLEEIVKEQVDQFPVERLEEMVLSISKKEFKMITYLGGLLGGIIGAIQALFVILF 4QIK Chain:A ((4-313)) ----------------GVEDTKHYEEAKKCVEELALYLKPLSSARGVGLNSTTQSVLSRPMQRKLVTLVHCQLVEEEGRIR---AMRAARSLGERTVTELILQHQ-NPQQLSSNLWAAVRARGCQ----FLGPAMQEEALKLVLLALEDGSALSRKVLVLFVVQRLEPRFPQAS------------------------------KTSIGHVVQLLYRASCFKSLMQLKEEFR------TYEALRREHDSQIVQIAMEAGLRIAPDQWSSLLYGDQSHKSHMQSIIDKLQTPASFAQSVQELTIALQRT---------------GDPANLNRLRPHLELLANIDPSPDAPP---------PTWEQLENGLVAVRTVVHGLVDYIQNH-----

General information: TITO was launched using: RESULT: Template: 4QIK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1184 -101717 -85.91 -353.18 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -85.91 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.329

(partial model without unconserved sides chains): PDB file : Tito_4QIK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4QIK-query.scw PDB file : Tito_Scwrl_4QIK.pdb: