Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLLSKKSEYKTLSTVEHPQYIVFCDFDETYFPHTIDEQKQQDIYELEDYLEQKSKDGELIIGWVTGSSIESILDKMGRGKFRYFPHFIASDLGTEITYFSEHNFGQQDNKWNSRINEGFSKEKVEKLVKQLHENHNILLNPQTQLGKSRYKHNFYYQEQDEINDKKNLLAIEKICEEYGVSVNINRCNPLAGDPEDSYDVDFIPIGTGKNEIVTFMLEKYNLNTERAIAFGDSGNDVRMLQTVGNGYLLKNATQEAKNLHN-------LITDSEYSKGITNTLKKLIGS 1S2O Chain:A ((2-242)) -----------------RQLLLISDLDNTWVGDQQ------ALEHLQEYLGDR--RGNFYLAYATGRSYHSARELQKQVGLM-EPDYWLTAVGSEIYHPE--GL---DQHWAD----YLSEHWQRDILQAIADGFE-ALKPQSPLEQNPWKISYHLDPQAC---PTVIDQLTEMLKETGIPVQVI--------FSSGKDVDLLPQRSNKGNATQYLQQHLAMEPSQTLVCGDSGNDIGLFETSARGVIVRNAQPELLHWYDQWGDSRHYRAQSSHAGAILEAIAHFDF-

General information: TITO was launched using: RESULT: Template: 1S2O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1240 -107681 -86.84 -460.18 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -86.84 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.443

(partial model without unconserved sides chains): PDB file : Tito_1S2O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1S2O-query.scw PDB file : Tito_Scwrl_1S2O.pdb: