Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEHQVRCAVLGLGRLGYYHAKNLVTSVPGAKLVCVGDPLKGRAEQVARELGIEKWSEDPYEVLEDPGIDAVIIVTPTSTHGDMIIKAAENGKQIFVEKPLTLSLEESKAASEKVKETGVICQVGFMRRFDPAYADAKRRIDAGEIGKPIYYKGFTRDQGA----PPAEFI---KHSGGIFIDCSIHDYDIARYLLGAEITSVSGHGRILNNPFMEQYGDVDQALTYIEFDSGAAGDVEASRTSPYGHDIRAEVIGTEGS-IFIGTLR---HQH----V-TILSAKG-SSFDI----IPDFQTRFHEAYCLELQHFAECVRNGKTPI-VTDIDATINLEVGIAATNSFRNGMPVQLDVKRAYTGM 3DB2 Chain:A ((2-352)) MYNPVGVAAIGLGRWAYVMADAYTK-SEKLKLVTCYSRTEDKREKFGKRYNC-AGDATMEALLAREDVEMVIITVPNDKHAEVIEQCARSGKHIYVEKPISVSLDHAQRIDQVIKETGVKFLCGHSSRRLGALRKMKEMIDTKEIGEVSSIEAVFSNERGLELKKGNWRGEPATAPGGPLTQLGVHQIDNLQFLLGP-VARVFNFGKPM-YTE---VENITVNQTLLEFEDGKQAYLGTNWACPGV--FSINVYGTKANLFYQLDFSWWSNSDVTDEHSTLIKREFAS---NRILRDVKVDFESVDHLRVEVEEVADVIRNGG-ETEIGAEASLRNLAVVLAAVKSVHEKRPVEIAEI------

General information: TITO was launched using: RESULT: Template: 3DB2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1906 -178765 -93.79 -553.45 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -93.79 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.467

(partial model without unconserved sides chains): PDB file : Tito_3DB2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DB2-query.scw PDB file : Tito_Scwrl_3DB2.pdb: