Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIGIVGATGYGGTELVRILSHHPHAEECILYSSSGEGNVYSEGYPHLTGLADQQLKPID--M-NTIKHEIDIMFLAAPPGVSSELTPKLADAGITVIDLSGDLRIKEPAEYEKWYKRTAA-PKAVIQEAVYGLAELNQLQIQQAKLIANPGCFPTAVLLGLAPLAQKKLLDESFVIVDAKTGVSGAGRK-ASMGTHFSELNDNFKIYKV-NEHQHTPEIEQALNEWQPGLGPITFSAHLVPMTRGIMATMYTRLTCDLTADDLHDLYSEFYQDSYFVRVRPKGQYPQTKEVYGSNFCDIAVTLDERTNRVTIVSVIDNLMKGAAGQAVQNFNLMNGWNEETGLTITPIYP 1VKN Chain:A ((2-339)) IRAGIIGATGYTGLELVRLLKNHPEAKITYLSSRTYAGKKLEEIFPSTLE-----NSILSEFDPEKVSKNCDVLFTALPAGASYDLVREL--KGVKIIDLGADFRFDDPGVYREWYGKELSGY-ENI-KRVYGLPELHREEIKNAQVVGNPGCYPTSVILALAPALKHNLVDPETILVDAKSGVSGAGRKEKVDYLFSE-VNESLRPYNVAK-HRHVPEMEQELGKISGKKVNVVFTPHLVPMTRGILSTIYVKTDK--SLEEIHEAYLEFYKNEPFVHVLPMGIYPSTKWCYGSNHVFIGMQMEERTNTLILMSAIDNLVKGASGQAVQNMNIMFGLDETKGLEFTPIYP

General information: TITO was launched using: RESULT: Template: 1VKN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1989 -187813 -94.43 -565.70 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -94.43 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.439

(partial model without unconserved sides chains): PDB file : Tito_1VKN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VKN-query.scw PDB file : Tito_Scwrl_1VKN.pdb: