Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKKRVVVTGLGALSPLGNDVDTSWNNAINGVSGIGPITRVDAEEYPAKVA-----AELKDFNVEDYM---DKKEARKMDRFTQYAVVAAKMAVEDADLNITDEIA-PRVGVWVGSGIGGLETLESQFEIFLTKGPRRVSPFFVPMMIPDMATGQISIALGAKGVNSCTVTACATGTNSIGDAFKVIQRGDADVMVTGGTEAPLTRMSFAGFSANKALST--NPDPKTASRPFDKNRDGFVMGEGAGIIVLEELEHALARGAKIYGEIVGYGSTGDAYHITAPAQDGEGGARAMQEAIKDAGIAPEEIDYINAHGTSTYYNDKYETMAIKTVFGEHAHKLAVSSTKSMTGHLLGAAGGIEAIFSILAIKEGVIPPTINIQTPDEECDLDYVPDEARRQELNYVLSNSLGFGGHNATLIFKKYQS 4JGA Chain:B ((16-432)) ---KRVVITGLGLVTPVGLNVNSSWKNIVDGVSGIKTITEFDTSKLACKIAGLIDNSEKDGFKLENFTQADDINRLSKMDKFIHYGVAAATEAVEDSGWLPDDEKSRDRTGLILGSGIGGLKMIEDTSIKLYQENNGKVSPFFIPASLINLLSGLVSIKYGFSGPNQTAVTACSTGAHAIGDAMRMIKHGYADVMIAGGAEAPVTPVGVAGFVAARALCTKYNDNPKKASRPWDKDRSGFVMGEGAGVVVLEEYEHALNRGAKVYGEVIGYGSTGDAYHMTAPHPEGRGAYRAMRDAMLDATITPDMIDYINAHGTSTTLGDGIELAAVQKLFLEANPKVLMSSTKSSIGHLLGAAGSVEFIFSALAIRDQIAPPTLNLDTPMDEVNIDLVALKAKKTKIDYVLSNSFGFGGTNASLVIK----

General information: TITO was launched using: RESULT: Template: 4JGA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2846 -208478 -73.25 -513.49 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain B : 0.88 3D Compatibility (PKB) : -73.25 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.499

(partial model without unconserved sides chains): PDB file : Tito_4JGA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JGA-query.scw PDB file : Tito_Scwrl_4JGA.pdb: