Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVTLYTSPSCTSCRKARAWLEEHEIPFVERNIFSEPLSIDEIKQILRMTEDGTDEIISTRSKVFQKLNVN--VESMPLQDLYRLINEHPGLLRRPIIIDEKRLQVGYNEDEIRRFLPRKVRSFQLREAQRLAN 3GKX Chain:A ((3-117)) -TLFLQYPACSTCQKAKKWLIENNIEYTNRLIVDDNPTVEELKAWIPLSGLPVKKFFNTSGVVYKELKLSSKLPTMTEEEQIALLATNGKLVKRPLVVTERFVLVGFKPEEWEKLK-----------------

General information: TITO was launched using: RESULT: Template: 3GKX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 473 -65807 -139.13 -582.36 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -139.13 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.663

(partial model without unconserved sides chains): PDB file : Tito_3GKX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GKX-query.scw PDB file : Tito_Scwrl_3GKX.pdb: