Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDYLSLSLTMIFVLIALFLSKSFKAGVEKDMIIATIRAAVQLLIIGYVLSLIFRGDHPVFILLMVLLMLAVAAQNVIKRKKNTIGSFWRVFAALAIVEIVTQGILLSLHIIPLTARYVIPISGMVIGNS--------MVLSSLFLNRLNSEVGVRKEEIQLILSLGGTPKQSIQRILTSAMKMSMIPTLESQKTLGLVQLPGMMTGQILAGADPIQA-VRFQLLIVFT-----TMASAALTCVILSVLTYPSLFTVHQQLKQNE 1N6V Chain:A ((1-212)) ---------------------SYDSPDYTDE-----SCTFKISLRNFRSILSWELKNHSIVPTHYTLLYTIMSKPEDLKVVKNCAN------------TTRSFCDLTDEWRSTHEAYVTVLEGFSGNTTLFSCSHNFWLAIDMSFEPPEFEIVGFTNHINVMVKFPSIVEEELQFDLSLVIEEQSEGIVKKHKP----EIKGNMSGNFTYIIDKLIPNTNYCVSVYLEHSDEQAVIKSPLKCTLLP----------PGQESEFS

General information: TITO was launched using: RESULT: Template: 1N6V.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 907 -149139 -164.43 -753.23 target 2D structure prediction score : 0.20 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -164.43 2D Compatibility (Sec. Struct. Predict.) : 0.20 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.166

(partial model without unconserved sides chains): PDB file : Tito_1N6V.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1N6V-query.scw PDB file : Tito_Scwrl_1N6V.pdb: