Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MERFEITKTPINTENIIKKVEKR-EAGAITTFIGTVREWTNGKRTVRLEYEAYEPMAVQMLAQIGAEIEEKWEGASAAITHRIGVLDIGEAAVVIAVSSPHRKAAYEANEYAIERIKQIVPIWKKEIWEDGEQWIGDQLETTAYPNGKPDLSEGEQHD 1FM0 Chain:E ((2-150)) AETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVR-------VNALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSAHRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWV---------EARESDQQAAKRW-

General information: TITO was launched using: RESULT: Template: 1FM0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 597 -63928 -107.08 -456.63 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain E : 0.75 3D Compatibility (PKB) : -107.08 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.517

(partial model without unconserved sides chains): PDB file : Tito_1FM0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FM0-query.scw PDB file : Tito_Scwrl_1FM0.pdb: