Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRVISGSKKGRSLKAVAGTSTRPTTDKVKESIFNMIGPYFDGGRGLDLFAGSGGLGIEALSRGFEHCIFVDRDFKAIQTVKSNLKTLELT-KHAQVYRNDAERALHAAAKRETGFRGIFLDPPYKEQK--------LKALLTLIDEYQM-LEEDGFIVAEHDREVELPETVGDLVMTRKETYGLTGVAIYKKRG 2IGT Chain:A ((153-276)) ----------------------------------------RPLKVLNLFGYTGVASLVAAAAGA-EVTHVDASKKAIGWAKENQVLAGLEQAPIRWICEDAMKFIQREERRGSTYDIILTDPPKFGRGTHGEVWQLFDHLPLMLDICREILSPKALGLVLTAYSI-----------------------------

General information: TITO was launched using: RESULT: Template: 2IGT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 538 -49712 -92.40 -436.07 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -92.40 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.565

(partial model without unconserved sides chains): PDB file : Tito_2IGT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2IGT-query.scw PDB file : Tito_Scwrl_2IGT.pdb: