Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MPEGKIIKALSGFYYVLDESEDSDKVIQCRGRGIFRKNKITPLVGDYVVYQAENDKEGYLMEIKERTNELIRPPICNVDQAVLVFSAVQPSFSTALLDRFLVLVEANDIQPIICITKMDLIEDQDTEDTIQAYAEDYRNIGYDVYLTSSKDQDSLADIIPHFQDKTTVFAGQSGVGKSSLLNAISPELGLRTNEISEHLGRGKHTTRHVELIH-TSGGLVADTPGFSSLEFTDIEEEELGYTFPDIREKSSSCKFRGCLHLKEPKCAVKQAVEDGELKQYRYDHYVEFMTEIKDRKPRY 1U0L Chain:A ((9-299)) LRRRGIVVSFHSNMVTVEDE--ETGERILCKLRGKFRLQNLKIYVGDRVEYTPDETGSGVIENVLHRKNLLTKPHVANVDQVILVVTVKMPETSTYIIDKFLVLAEKNELETVMVINKMDLYDEDDLRKVRELEEIY--SGLYPIVKTSAKTGMGIEELKEYLKGKISTMAGLSGVGKSSLLNAINP-------------GLKLRTTTTAQLLKFDFGGYVVDTPGFANLEINDIEPEELKHYFKEFGD--KQCFFSDCNHVDEPECGVKEAVENGEIAESRYENYVKMFYELLGRR---

General information: TITO was launched using: RESULT: Template: 1U0L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1414 -155251 -109.80 -562.50 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -109.80 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.491

(partial model without unconserved sides chains): PDB file : Tito_1U0L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1U0L-query.scw PDB file : Tito_Scwrl_1U0L.pdb: