Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTQFDKQYNSIIKDIINNGISDEEFDVRTKWDSDGTPAHTLSVISKQMRFD-NSEVPILTTKKVAWKTAIKELLWIWQLKSNDVNDLNMMGVHIWDQ------------WKQEDGTIGHAYGFQ---LGKKNRSL----NGEKVDQVDYLLHQLKNNPSSRRHITMLWNPDELDAMALTPCVYETQWYVKHGKLHLEVRARSNDMALGNPFNVFQYNVLQRMIAQVTGYELGEYIFNIGDCHVYTRHIDNLKIQMEREQFEAPELWINPEVKDFYDFTIDDFKLINYKHGDKLLFEVAV 1JUJ Chain:D ((32-313)) ------QYLGQIQHILRCGVEKD----------DRTGTGTLSVFGMQARYSLRDEFPLLTTKRVFWKGVLEELLWFIK-GSTNAKELSSKGVKIWDANGSRDFLDSLGFSTREEGDLGPVYGFQWRHFGAEYRDMESDYSGQGVDQLQRVIDTIKTNPDDRRIIMCAWNPRDLPLMALPPCHALCQFYVVNSELSCQLYQRSGDMGLGVPFNIASYALLTYMIAHITGLKPGDFIHTLGDAHIYLNHIEPLKIQLQREPRPFPKLRILRKVEKIDDFKAEDFQIEGYNPHPTIKMEMAV

General information: TITO was launched using: RESULT: Template: 1JUJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1252 8877 7.09 33.88 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain D : 0.78 3D Compatibility (PKB) : 7.09 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.557

(partial model without unconserved sides chains): PDB file : Tito_1JUJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1JUJ-query.scw PDB file : Tito_Scwrl_1JUJ.pdb: