Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIRKILLSSALSFGMLISAVPALAAGTSSEVAVKNEEYDYDTIYRISPLPMDFQPTIEYNGYTYTLTRHYFDYSIGFYTAIYTKVV 1XOU Chain:B ((2-85)) -GIVSQTRNKELLDKKIRSEIEAIKKIIAEFDVVKESVNELSEKAKTDP-QAAEKLNKLIEGYTYGEERKLYDSALSKIEKLIETL-

General information: TITO was launched using: RESULT: Template: 1XOU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 78 -1548 -19.85 -18.43 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain B : 0.59 3D Compatibility (PKB) : -19.85 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.59 QMean score : -0.173

(partial model without unconserved sides chains): PDB file : Tito_1XOU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1XOU-query.scw PDB file : Tito_Scwrl_1XOU.pdb: