Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEFHDDKKNELQKKEEIITEAIDTLFQSSAFGNLINGFQNLINSSLKDVQTTIHVRERDTGLYIDITIPATFRDGEIVVDVKSRYLHVTLQEKQKHQNEATFTSMTRTVQLPYEVRQEDMETSW-NEQTMTLFFPKNKHE 4YE0 Chain:A ((2-89)) --------------------------------------------------SLNEVENTAQKFCVKLDVAAFKP-EELKVNLEGHVLTIEGHHEVKTEHGFSKRSFTRQFTLPKDVDLAHIHTVINKEGQMTIDAPKTGS-

General information: TITO was launched using: RESULT: Template: 4YE0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 341 -29558 -86.68 -339.74 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -86.68 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.425

(partial model without unconserved sides chains): PDB file : Tito_4YE0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YE0-query.scw PDB file : Tito_Scwrl_4YE0.pdb: