Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRQITDISQLKEAIKQYHSEGKSIGFVPTMGFLHEGHLTLADKA-RQENDAVIMSIFVNPAQFGPNEDFEAYPRDIERDAALAENAGVDILFTPDAHDMYPGEKNVTIHVERRTDVLCGRSREGHFDGVAIVLTKLFNLVKPTRAYFGLKDAQQVAVVDGLISDFFMDIELVPVDTVREEDGLAKSSRNVYLTAEERKEAPKLYRALQTSAELVQAGERDPEAVIKAAKDIIETTSGT-IDYVELYSYPELEPVNEIAGKMILAVAVAFSKARLIDNIIIDIREMERI 4MUN Chain:A ((19-287)) -----DVADVSRALRL---TGRRVMLVPTMGALHEGHLALVRAAKRVPGSVVVVSIFVNPMQFG---DLDAYPRTPDDDLAQLRAEGVEIAFTPTTAAMYPDGLRTTVQPGPLAAELEGGPRPTHFAGVLTVVLKLLQIVRPDRVFFGEKDYQQLVLIRQLVADFNLDVAVVGVPTVREADGLAMSSRNRYLDPAQRAAAVALSAALTAAAHAATAGA---QAALDAARAVLDAAPGVAVDYLELRDI-GLGPM-PLNGSGRLLVAARLGTTRLLDNIAIEI------

General information: TITO was launched using: RESULT: Template: 4MUN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1358 -78955 -58.14 -299.07 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -58.14 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.507

(partial model without unconserved sides chains): PDB file : Tito_4MUN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MUN-query.scw PDB file : Tito_Scwrl_4MUN.pdb: