Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---MIRGILIAVLGIAIVGTGYWGYK-EHQEKDAVLLHAENNYQRAFHELTYQVDQLHDKIGTTLAMNSQKSLSPALIDVWRITS-EAHNSVSQ-LPLTLMPFNKTEELLSKIGDFS---------YKTSVRDLDQKPLDKNEYTSLNKLYQQSEDIQNELRHVQHLVMSKNLRWMDVEMALASDEKQSDNTIINSF-KTVEKNVGAFSTGTDLGPSFTSTKKEEKGFSHLKGKQISEQEAKQIAERFAPDDNYSIKVVKSGKKTNRD---------VYSISMKDPD-HKAVIYMDITKKGGHPVYLIQNREVKDQKISLNDGSNRALAFLKKN----GFETDD-LEIDESAQYDKIGVFSYVPVENKVRMYPEAIRMKVALDDGEVVG----FSARDFLTSHRKRTIPKPAITEAEAKSKLNKNVQVRETRLALITNELGQEV-----LCYEMLGTIENDTFRMYINAKDGSEEKVEKLKNAEPIYKDL 5DIY Chain:B ((2-473)) EYFRYRGIIE----------GFYGKPWEHQERLDMFEFMQANNLNAYIYAPKQ-DLYHRELWREPYKEEQLQLFKELIEKAGSCGINFTFAISPGLSLVYSSEEELETLIRKITPFLEMGVHSIGIFFDNVPFDLIHEEDRNSYSNL--AEAQADFLTRVLQRLESTIST-----PQIIMCPTFYCNDPNLEYLRILGQRLPKNIDVFWTGPNVC-SHEIT------TSHMQEVQKSLQRPATLWDNYPVNDGGMMPELHIGPYDHRDPELHTHVVGIYANPMALPEASKLPLYTFAQYLNSPSQYNPQDSWRQAVSTLLGEDNLSAMEKFYQSNTISCLEPEEPAYLTNLFKKVQEDFASFRFEQGLRTLREEIISMQTTYSRLSTQDSKFFWEIRPWLEEYKLWTDYLDQAMITFSNLFARESLQKALQGRTYLREVLKDAVDFRTRVCGDVVRNFLQQVLRSTVSIELQAEGKEWTALPPGIVR---

General information: TITO was launched using: RESULT: Template: 5DIY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2100 96920 46.15 229.67 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain B : 0.63 3D Compatibility (PKB) : 46.15 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.108

(partial model without unconserved sides chains): PDB file : Tito_5DIY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DIY-query.scw PDB file : Tito_Scwrl_5DIY.pdb: