Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNEKNIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHFTLELVQR----CNFVTAIEIDHKLCKTTENKLVDHDNFQVLNKDILQFKFP------KNQSYKIFGNIPYNISTDIIRKIVFDSI-ADEIYLIVEYGFAKRLLN--T---KRSLALFLMAEVDISILSMVPREYFHPKPKVNSSLIRLNRKKS-RISHKDKQKYNYFVMKWVNKE---YKKIFTKNQFNNSLKHAGI---DDLNNISFEQFLSLFNSYKLFNK 3UZU Chain:A ((18-276)) -----KRFGQNFLVDHGVIDAIVAAIRPERGERMVEIGPGLGALTGPVIARLATPGSPLHAVELDRDLIGRLEQRF-G-ELLELHAGDALTFDFGSIARPGDEPSLRIIGNLPYNISSPLLFHLMSFAPVVIDQHFMLQNEVVERMVAEPGTKAFSRLSVMLQYRYVMDKLIDVPPESFQPPPKVDSAIVRMIPHAPHELPAVDPAVLGEVVTAAFSQRRKMLRNTLGGYRDLVDFDALGFDLARRAEDIGVDEYVRVAQAVASAR-

General information: TITO was launched using: RESULT: Template: 3UZU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1064 8670 8.15 36.74 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 8.15 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.531

(partial model without unconserved sides chains): PDB file : Tito_3UZU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3UZU-query.scw PDB file : Tito_Scwrl_3UZU.pdb: