Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDPSTLMILAIVAVAIIVLAVFFTFVPVMLWISALAAGVKISIFTLVGMRLRRVIPNRVVNPLIKAHKAGLNVGTNQLESHYLAGGNVDRVVNALIAAQRANIELTFERCAAIDLAGRDVLEAVQMSVNPKVIETPFIAGVAMDGIEVKAKARITVRANIERLVGGAGEETIVARVGEGIVSTIGSSDNHKKVLENPDMISQTVLGKGLDSGTAFEILSIDIADVDIGKNIGAILQTDQAEADKNIAQAKAEERRAMAVAQEQEMRARVEEMRAKVVEAEAEVPLAMAEALREGNIGVMDYMNIKNIDADTEMRDSFGKLTKDPSDEDRKS 4FM3 Chain:A ((45-93)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------MTAESYKKARLLAEQAELDARLAESKVLTQKSKDQ--LGELDKSLKRLRKQ--------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4FM3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 13 -1235 -95.00 -25.20 target 2D structure prediction score : 0.82 Monomeric hydrophicity matching model chain A : 0.50 3D Compatibility (PKB) : -95.00 2D Compatibility (Sec. Struct. Predict.) : 0.82 1D Compatibility (Hydrophobicity) : 0.50 QMean score : 0.760

(partial model without unconserved sides chains): PDB file : Tito_4FM3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FM3-query.scw PDB file : Tito_Scwrl_4FM3.pdb: