Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFTAYQDARSWIHGRLKFGVKPGLGRMKQLMARLGHPEKKIRAFHVAGTNGKGSTVAFIRSMLQEAGYTVGTFTSPYIITFNERISVNGIPISDEEWTALVNQMKPHVEALDQTEYGQPTEFEIMTACAFLYFAEFHKVDFVIFETGLGGRFDSTNVVEPLLTVITSIGHDHMNILGNTIEEIAGEKAGIIKE---GIPIVTAVTQPEALQVIRHEAERHAAPFQSLHDA-CVIFNEEA---LPAGEQFSFKTEEKCYEDIRTSLIGTHQRQNAALSILAAEWLNKENIAHISDEALRSGLVK-AAWPGRLELVQE------HPPVYLDGAHNEEGVEKLAETMKQRFANSRISVVFSALKDKPYQN----MIKRLETIAHAIHFASFDF--P------RASLAKDLYD-A---SEISNKSWSEDPDDVIKFIESKKGSNEIVLITGSLYFISDIRKRLK 1P3D Chain:A ((117-465)) ---------------------------------------FRHGIAVAGTHGKTTTTAMISMIYTQAKLDPTF--------------VNGGLVKSA--------------------GK--N------------AHLG-ASRYLIAEADESDA--SFLHLQPMVSVVTNMEPDHMDTYEGDFEKMKATYVKFLHNLPFYGLAVMCADDPVLMELVP-K---VGRQVITYGFSEQADYRIEDYEQTGFQGHYTVICPNNERINVLLNVPGKHNALNATAALAVAKEEG------IANEAILEALADFQGAGRRFDQLGEFIRPNGKVRLVDDYGHHPTEVGVTIKAAREGWGDKRIVMIFQPHRYSRTRDLFDDFVQVLSQ-VDALIMLDVYAAGEAPIVGADSKSLCRSIRNLGKVDPILVSDTSQLGDV----LDQIIQDGDLILAQGAGSVSKI------

General information: TITO was launched using: RESULT: Template: 1P3D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1813 -5527 -3.05 -17.32 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -3.05 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.386

(partial model without unconserved sides chains): PDB file : Tito_1P3D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1P3D-query.scw PDB file : Tito_Scwrl_1P3D.pdb: