Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGTNVQVDSASAECTQTMSGALMLIESLKKEKVEMIFGYPGGAVLPIYDKLYNS-GLVHILPRHEQGAIHAAEGYARVSG--KPGVVIATSGPGATNLVTGLADAMIDSLPLVVFTGQVATSVI------GSDAFQEADILGITMPVTKHSYQVRQPEDLPRIIKEAFHIATTGRPGPVLIDIPKDVATIEGEFSYDHEMNLPGYQPTTEPNYLQIRKLVEAVSSAKKPVILAGAGVLHGKASEELKNYAEQQQIPVAHTLLGLGGFPADHPLFLGMAGMHGTYTANMALHECDLLISIGARFDDRVTGNL-KHFARNAKIAHIDIDPAEIGKIMKTQIPVVGDSKIVLQELIKQDGKQS-DSSEWKKQLAEWKEEYPLW-YVDNEEEGFKPQKLIEYIHQFTKGEA-IVATDVGQHQMWSAQFYPFQKADKWVTSGGLGTMGFGLPAAIGAQLAEKDATVVAVVGDGGFQMTLQELDVIRELNLPVKVVILNNACLGMVRQWQEIFYEERYSESKF-ASQPDFVKLSEAYGIKGIRISSEAEAKEKLEEALTSREPVVIDVRVASEEKVFPMVAPGKGLHEMVGVKP 5AHK Chain:A ((21-573)) -----------------MKASDAVAKILADNNVLYGFELIGGMITHLVDSINLLGKTKLVSMHHEQGAAFAASAVSRVTHHKTLGLALATSGPGATNLITGIADCWLDSHPCIFLTGQVNTHELKGKRDIRQQGFQELDSVALVTSITKYAYQIKSADELVPCLRKAIQIAKEGRPGPVLLDIPMDIQRADIDEALLNN---------PSIAMSDLDFIINKLQNAKKPLLLIGGGAVNSSGFQKWLEQIELRGIPYVASLKGAEKIKA-SDLYLGMLGAYGTRAANHAVQNCDLLLVLGSRMDVRQTGAQPEDFARNAEIIQIDLQEGQLNNRVIADFSYQIELSEYFSRFSPLQIPVNNDWSVWTALLKE---KFRVTFIDEYTTWNLSPFGLFTQLNKLTERVALDYILDVGNNQMWAAHTLRLNAQQAMHHSGGLGSMGFAIPAAIGACYAG-KKPIIVITGDGGAQLNIQELDIIARDKLPILTIVMNNHSLGMVRGFQEMYFEGRNSSTYWNGYTSQFKKIGEAYRVESKTIISMQAFSSALESFLESPRPLLLEVSMSDARECRPRL--------------

General information: TITO was launched using: RESULT: Template: 5AHK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3308 -19582 -5.92 -37.02 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -5.92 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.516

(partial model without unconserved sides chains): PDB file : Tito_5AHK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5AHK-query.scw PDB file : Tito_Scwrl_5AHK.pdb: