Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTRADFIQFGAMIHGVGGTTDGWRHPDVDPSASTNIEFYMKKAQTAEKGLFSFIFIADGLFISEKSIPHFLNRFEPITILSALASVTKNIGLVGTFS-TSFTEPFTISRQLMSLDHISGGRAGWNLVTSPQEGAARNHSKSNLPEHTERYEIAQEHLDVVRGLWNSWEHDAFIHNKKTGQFFDQAKLHRLNHKGKYF-QVEGPLNIGRSKQGEPVVFQAGS-SETGRQFAAKNADAIFTHSN--SLEETKAFYADVKSRAADEGRDPSSVRIFPGISPIVADTEEEAEKKYREFAELIPIENAVTYLARFFDDYDLSVYPLDEPFPDIGDVGKNAFQSTTDRIKREAKARNLTLREVAQEMAFPRTLFIGTPERVASLIETWFNAEAADGFIVGSD--------IPGTLDAFVEKVIPILQERGLYRQDYRGGTLRENLGLGIPQHQSVLHSSHH 2I7G Chain:A ((21-364)) -----GMELGLYTFADVNPNPADGRG---PEGARRLRELLEEIELADQVGLDVFGLGEHHRPDY-------VVSSPSTVLAAAAVKTKNIRLTSAVSVLSSDDPVRVFQQFSTVDLLSNGRAEIMAGRGSFIESYPLFGY----DLEDYDVLFAEKLDLLLALREQ---------------------EVVTWSGTKHPAINGRGVYPRPLQERLPVWIAVGGTPQSVARAGAMGLPVALAIIGGEYRRFAPLFDLYHEAARRAGQEKTKLRTSINVHGFIADTTDKAADQFYGPQAEVMN-----RIGRERG------WGPT---N----------------------R--AHFDAA---RGPEGNLFLGEPELVAEKIIKAHGVFKNDRFLLQMAIGLMPHDQIMRGIELYGTKVAPLVRKELT------------------------------

General information: TITO was launched using: RESULT: Template: 2I7G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1776 -3280 -1.85 -9.91 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -1.85 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.497

(partial model without unconserved sides chains): PDB file : Tito_2I7G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2I7G-query.scw PDB file : Tito_Scwrl_2I7G.pdb: