Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTDQRRNVYFFHKQDQETNEQARSLTQLAEEHGFTVVNQHSDANIIASIGGDGTFLQAVRKTNF--RDDCLYVGITKKGKAHLYCDFHSDEREKMVDAMTFEQIEVRKYPLIEVTVDQ-----ASPFHCLNEVSIRSSIIKTFVMDVLIDDLHFETFRGDGMIISTPTGSTAYNKSVAGAVVDPLLPCMQVSELASLNNNTYRTLGSPFVLSSDRKLTLRVVQDGNEHPIIGLDNEALSTRNVKTIEIKLSNKKIKTVKLKDNSFWEKVKRTFL 2I2C Chain:A ((1-262)) -----MKYMITSKGDEKSDLLRLNMIAGFGEYDMEY--DDVEPEIVISIGGDGTFLSAFHQYEERLD-EIAFIGIH-TGHLGFYADWRPAEADKLVKLLAKGEYQKVSYPLLKTTVKYGIGKKEATYLALNESTVKSS-GGPFVVDVVINDIHFERFRGDGLCMSTPSGTTAYNKSLGGALMHPSIEAMQLTEMASINNRVYRTIGSPLVFPKHHVVSLQPVND--KDFQISVDHLSILHRDVQEIRYEVSAKKIHFARFRSFPFWRRVHDSFI

General information: TITO was launched using: RESULT: Template: 2I2C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1391 -1234 -0.89 -4.84 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : -0.89 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.458

(partial model without unconserved sides chains): PDB file : Tito_2I2C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2I2C-query.scw PDB file : Tito_Scwrl_2I2C.pdb: