Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTHIYKKLGAAFFALLLIAALAACGNNSESKGSASDSKGAETFTYKAENGNVKIPKHPKRVVVMADGYYGYFKTLGINVVGAPENVFKNPYYKGK--TNGVENIGD--GTSVEKVIDLNPDLIIVWTTQGADIKKLEKIAPTVAVKYDK-----LDNIEQLKEFAKMTGTEDKAEKWLAKWDKKVAAAKTKIKKAVGDKTISIMQTNGKDIYVFGKDFGRGGSIIYKDLGLQATKLTKEKAIDQGPGYTSISLEKLPDFAGDYIFAGPWQSGGDDGGVFESSIWKNLNAVKNGHVYKMDPIGFYFTDPISLEGQLEFITESLTK 1EFD Chain:N ((2-263)) ------------------------------------------------------GIDPNRIVALEWLPVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVWSAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYEDFIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPN--SLFQEILDEYGIPNAWQGET----NFWGSTAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAV-WFYGATLSAMHFVRVLDNAIG-

General information: TITO was launched using: RESULT: Template: 1EFD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain N - contact count / total energy / energy per contact / energy per residue : 1333 12229 9.17 48.33 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain N : 0.70 3D Compatibility (PKB) : 9.17 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.538

(partial model without unconserved sides chains): PDB file : Tito_1EFD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EFD-query.scw PDB file : Tito_Scwrl_1EFD.pdb: