Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNQLIQAQKKLLPDLLLVMQKRFEILQYIRLTEPIGRRSLSASLGISERVLRGEVQFLKEQNLVDIKTNGMTLTEEGYELLSVLEDTMKDVLGLTLLEKTLKERLNLKDAIIVSGDSDQSPWVKKEMGRAAVACMKKRFSGKNIVAVTGGTTIEAVAEMMTPDSKNRELLFVPARGGLGE--DVKNQANTICAHMAEKASGTYRLLFVPGQLSQG-AYSSIIEEPSVKEVLNTIKSASMLVHGIGEAKTMA--QRRNTPLED-LKKIDDNDAVTEAFGYYFNADGEVVHK---VHSVGMQLDDIDAIPDIIAVAGGSSKAEAIEAYFKKPR-NTVLVTDEGAAKKLLRDE 2W48 Chain:A ((8-315)) -------------------RLIVKIAQLYYE-QDMTQAQIARELGIYRTTISRLLKRGREQGIVTIAINY----------------DY---NENLWLEQQLKQKFGLKEAVVASS----EEEQLSAMGQHGALLVDRLLEPGDIIGFSWGRAVRSLVENLPQRSQSRQVICVPIIGGPSGKLESRYHVNTLTYGAAARLKAESHLADFPALLDNPLIRNGIMQSQHFKTISSYWDSLDVALVGIGSPAIRDGANWHAFYGSEESDDLNARHVAGDICSRFYDINGGLVDTNMSEKTLSIEMAKLRQARYSIGIAMGEEKYSGILGALHGRYIN-CLVTNRETAELLLK--

General information: TITO was launched using: RESULT: Template: 2W48.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1501 -88929 -59.25 -302.48 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -59.25 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.515

(partial model without unconserved sides chains): PDB file : Tito_2W48.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2W48-query.scw PDB file : Tito_Scwrl_2W48.pdb: