Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIGVIDYGMGNLYSVSKALERVGVPYFVSEK---P-EELKEADAFILPGVGSFGDAMDNLGYTKLDQLIHDMVSEGRLLLGICLGMQLLFEESEENGTASGLGLLKGKAVRLKAEDEKGNKLKVPHMGWNRLSFHNESPLLTKTE-QGYAYFVHSYYIDGMEENALLASADYGVRVP--AVVGKRNVFGAQFHPEKSSTVGMSILTQFTKMAAEQKVKK 1GPW Chain:B ((2-200)) RIGIISVGPGNIMNLYRGVKRASENFEDVSIELVESPRNDLYDLLFIPGVGHFGEGMRRLRENDLIDFVRKHVEDERYVVGVCLGMQLLFEESEEAPGVKGLSLIEGNVVKLRSR-------RLPHMGWNEVIF-------KDTFPNGYYYFVHTYRAV-CEEEHVLGTTEYD-GEIFPSAVRKGRILGFQFHPEKSSKIGRKLLEKVIECSLSR----

General information: TITO was launched using: RESULT: Template: 1GPW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1100 16510 15.01 85.99 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : 15.01 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.531

(partial model without unconserved sides chains): PDB file : Tito_1GPW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GPW-query.scw PDB file : Tito_Scwrl_1GPW.pdb: