Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTSQINRQYQLAQRPSGLPGRDTFSF--VETPLGEPAEGQILVKNEYLSLDPAMRGWMNDARSYIPPVGIGEVMRALGVGKVLVSKHPGFQAGDYVNGALGVQDYFIGEPKGFYKVDPSRAPLPRYLSALGMTGMTAYFALLDVGQPKNGETVVISGAAGAVGSVAGQIARLKGCRVVGIAGGAEKCRFLVEELGFDGAIDYKNE-DLAAGLKRECPKGIDVFFDNVGGEILDTVLTRIAFKARIVLCGAISQYNNKEAVRGPANYLSLLVNRARMEGMVVMDYAQRFPEGLKEMATWLAEGKLQSREDIVEGLETFPETLLKLFSGENFGKLVLKV 4HFN Chain:B ((7-340)) -----NKQVILKNYV-GYPKESDMEIKNVTIKLKVPGSNDVVVKNLYLSCDPYMRSRMRK-----ESFAPGSPITGYGVAKVLESGDPKFQKGDLVWGMTGWEEYSIITP--LFKIHDKDVPLSYYTGILGMPGMTAYAGFHEVCSPKKGETVFVSAASGAVGQLVGQFAKMLGCYVVGSAGSKEKVDLLKSKFGFDEAFNYKEEQDLSAALKRYFPDGIDIYFENVGGKMLDAVLVNMKLYGRIAVCGMISQ-------EGVHNLFCLITKRIRMEGFLVFDYYHLYPKYLEMVIPQIKAGKVVYVEDVAHGLESAPTALVGLFSGRNIGKQVVMV

General information: TITO was launched using: RESULT: Template: 4HFN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -182172 for 2727 contacts (-66.8/contact) + 2D Compatibility (PS) -34256 + (NN) -18926 + (LL) 1368 1D Compatibility (HY) -32400 + (ID) 6950 Total energy: -273336.0 ( -100.23 by residue) QMean score : 0.480

(partial model without unconserved sides chains): PDB file : Tito_4HFN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HFN-query.scw PDB file : Tito_Scwrl_4HFN.pdb: