Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKLIAIDMDGTLLNDEKKIPKENIQAIKEATQAGIKIVLCTGRPMSGILPYFNE-LGLTKEEYIIMNNGCSTYSTKDWQLIDSATLTHDELIFLEEVVKEFPNVCLTLTAENTFYAVGEEVPEIVAYDAD-LVFTKAKSTSLDALRNQEEIVFQAMYMGLDADVTAFQEAVEEALISKFSGVRSQDYIYEIMPQGVTKARGLKSLIAKLGLDINQVMAIGDAPNDIELLDLVPNSVAMGNASDEIKSRCKYITVDNNKAGVAKAIYDYALR 1NF2 Chain:A ((1-263)) MYRVFVFDLDGTLLNDNLEISEKDRRNIEKLSR-KCYVVFASGRMLVSTLNVEKKYFKR--TFPTIAYNGAIVYLPE-EGVILNEKIPPEVAKDIIEYIKPL-NVHWQAYIDDVLYSEKDNEE-IKSYARHSNVD-YRVEPNLSELVSKMG-TTKLLLIDTPERLDELKEILSERFKDVVKVFKSFPTYLEIVPKNVDKGKALRFLRERMNWKKEEIVVFGDNENDLFMFEEAGLRVAMENAIEKVKEASDIVTLTNNDSGVSYVLERISTD

General information: TITO was launched using: RESULT: Template: 1NF2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output 3D Compatibility (PKB) -173505 for 2193 contacts (-79.1/contact) + 2D Compatibility (PS) -28112 + (NN) -7199 + (LL) 404 1D Compatibility (HY) -14800 + (ID) 2750 Total energy: -225962.0 ( -103.04 by residue) QMean score : 0.485

(partial model without unconserved sides chains): PDB file : Tito_1NF2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1NF2-query.scw PDB file : Tito_Scwrl_1NF2.pdb: