Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVLKPG----------LLTTVQDIGRTGYQKYGVLASGAMDTVSLRIANLLIG-NGENEAGLEITMMGPGPSFHFSKQTLIAVTGADFTLRI-NDEEAPLWKPVLIKENSTVSFGPCKLGSRAYLAA------AGGIEVPA---------------VMESKSTYVRGSIGGLHGRALQKEDEL-----NIGEMSALSQTILSRLSS--QLGKQGFAAPKWSVSRG--------------------RFLPLKKNPVIRVLEGKQFAFFTEESKTRFYEEAFRV------TPQSDRMGYRLKGEPLELKAPLEMVSEAVSF-GTVQVPPDGNPIILLADRQTTGGYPRIAHIISADLPIVSQIMP--------GEHVQFEPVSLQEAEAL--AVEREQHIKEL-----------KTRMKMEWLT--------------------- 2BMF Chain:A ((169-618)) MASIEDNPEIEDDIFRKKRLTIMDLHPGAGKTKRYLPAIVREAIKRGLRTLILAPTRVVAAEMEEALRGLPIRYQTGREIVDLMCHATFTMRLLSPIRVPNYNLIIMDEAHFTD--PASIAARGYISTRVEMGEAAGIFMTATPPGSRDPFPQSNAPIMDEEREIPERSWNSGHEWVTDFKGKTVWFVPSIKAGNDIAACLRKNGKKVIQLSRKTFDSEYIKTRTNDWDFVVTTDISEMGANFKAERVIDPRRCMKPVILTDGEERVILAGPMPVTHSSAAQRRGRVGRNPKNENDQYIYMGEPLENDEDCAHWKEAKMLLDNINTPEGIIPSMFEPEREKVDAIDGEYRLRGEARKTFVDLMRRGDLPVWLAYRVAAEGINYADRRWCFDGVKNNQILEENVEVEIWTKEGERKKLKPRWLDARIYSDPLALKEFKEFAAGRK

General information: TITO was launched using: RESULT: Template: 2BMF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1345 -77794 -57.84 -233.61 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -57.84 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.149

(partial model without unconserved sides chains): PDB file : Tito_2BMF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2BMF-query.scw PDB file : Tito_Scwrl_2BMF.pdb: