Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKKWQWLVYGWKLIILTLLTAAVFSYAMFQGGFVSWFLFYAFLPFVLYAGLLALYPL---RSFQASRQ-----MDKT--QLHAGDRLGVTVT-LRRKLPFPLMYMVIEDCLPEGI-------ESLNR---------DGAAAKRLVFPWFKRSMTMSYELARV----PRGEHHFHSVRVRTGDVLGLIEKTAFFELDDTLFVYPFYQRFSYQVNERHQEDGVSGSSPIHQHHSSVAASVRNYQ-PGDRFAALDWKTSARRSQLMTKEFEPSRSKNLFLLMDRFSSDAFEEVVSVTASILHSVLKNGAGAGLASIGKEKNIFPIQEGDQHFKHMLRHLAIAHCDAADPISRY-AREELGKPSVRQADQVVVTGQLTE--DMLHLAEIGGGRVTVI-LAKEKDAELSQAENVMIERMMKRQ---------IRVRIMRGGRVSRVV----- 4Q6O Chain:A ((21-437)) MIEKAPTNVEDRDKAPHLLLLAGIQGDE--PGGFNATNLFLMH--YSVLKGLVEVVPVLNKPSMLRNHRGLYGDMNRKFAALDKNDPEYPTIQEIKSLIAKPSIDAVLHLHDGGGYYRPVYVDAMLNPKRWGNCFIIDQDEVKGAKFPNLLAFANNTIESINAHLLHPIEEYHLKNTRTAQGDT----------EMQKALTFYAINQKKSAFANEASKE--LPLASRVFYHLQAIEGLLNQLNIPFKRDFDLN---PNSVHALI-------NDKNLWAKISSLPK---MPLFNLRPKLNHFPLPHNTK--IPQIPIESNAYIVGLVKNKQEVFLKYGNKLMTRLSPFYIEFDPSLEEVKMQIDNKDQMVKIGSVVEVKESFYIHAMDNIRANVIGFSVSNENKPNEAGYTIKFKDFQKRFSLDKQERIYRIEFYKNNAFSGMILVKFV

General information: TITO was launched using: RESULT: Template: 4Q6O.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1888 -170431 -90.27 -464.39 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -90.27 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.152

(partial model without unconserved sides chains): PDB file : Tito_4Q6O.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Q6O-query.scw PDB file : Tito_Scwrl_4Q6O.pdb: