Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKRARIIYNPTSGREIFKKHLA-QVLQKFEQAGYETSTHATTCAGDATHAAKEAALREFDLIIAAGGDGTINEVVNGLAPLD-----NRPTLGVIPVGTTNDFARALGIPREDILKA--------A-DTVINGVARPIDIGQV---NG--QYFINIAGGGRLTELTYDVPSKLKTMLGQLAYYLKGMEMLPSLRPTEVEIEY-----------------------DGKL------F-QGEIMLFLVTLTNSVGGFEKLAPDSSLNDGMFDLMILKK-ANLAEFIRVATMALRGEHI--NDQHIIYTKANRVKVNVS-EKMQLNLDGEYGGMLPGEFVNLYRHIHVVMPKEKAEQLDD 4V24 Chain:A ((22-371)) PCRVLVLLNPRGGKGKALQLFRSHVQPLLAEAEISFTLMLTERRNHARELVRSEELGRWDALVVMSGDGLMHEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAG-YEQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEK--YRRLGEMRFTLGTFLRLAALRTYRGRLAYLPVGRVGSKTPASGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVS----------

General information: TITO was launched using: RESULT: Template: 4V24.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1676 -231438 -138.09 -798.06 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -138.09 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.384

(partial model without unconserved sides chains): PDB file : Tito_4V24.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4V24-query.scw PDB file : Tito_Scwrl_4V24.pdb: