Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNTYHPFSLTTPSTLMIQDWAQTNQNNKEVIAGFTTKNGGVSQKPFESLNTGLHVHDKDADVVKNREYIADMFNTDLQSWVFADQTHDNRVQKVTQRDRGKGAREYHTALKATDGIYTNEKNVFLALCFADCVPLFFYDPVKSLVGVAHAGWKGTVKQIGREMVKQWTEKEGSNPSDIYAVIGPSISGACYTVDDRVMDAVRALPVSADLAANQTAKAQYQLDLKELNRLILMDSGLASEQISVSGLCTESEPSLFYSHRRDQGKTGRMMSFIGMKEA 2NVM Chain:A ((46-64)) ---------------------------------------------------------------------------------------------------------------------------------------LIIDEQRDQYLWLCCGWDG----------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2NVM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 17 -6397 -376.29 -336.68 target 2D structure prediction score : 0.05 Monomeric hydrophicity matching model chain A : 0.55 3D Compatibility (PKB) : -376.29 2D Compatibility (Sec. Struct. Predict.) : 0.05 1D Compatibility (Hydrophobicity) : 0.55 QMean score : 0.971

(partial model without unconserved sides chains): PDB file : Tito_2NVM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2NVM-query.scw PDB file : Tito_Scwrl_2NVM.pdb: