Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------MNTLTVKDIKDRLQEVKDAQDPFIAQCENDPRKSVQTL------------VEQWLKKQAKEKALKEQWVNMTSYERLARNKGFRLIAGVDEVGRGPLAGPVVASAVILPEE----CEILGLTDSKKLSEKKREEYYELIMKEALAVGIGIVEATVIDEIN-----IYEASKMAMVKAIQDLSDT-----PDYLLVDAMTLPLDTAQ-------------ASIIKGDAKSVSIAAGACIAKVTRDRMMSAYAETYPMYGFEKNKGYGTKE-HLEALAAYGPTEL---HRKTFAPVQSFR------ 2D0B Chain:A ((2-309)) SNYVIQADQQLLDALRAHYEGALSDRLPAGALFAVKRPDVVITAYRSGKVLFQGKAAEQEAAKWISGASASNETADHQPSALAAHQLGSLSAIGSDEVGTGDYFGPIVVAAAYVDRPHIAKIAALGVKDSKQLNDEAIKRIAPAIMETV-PHAVTVLDNPQYNRWQRSGMPQTKMKALLHNRTLVKLVDAIAPAEPEAIIIDEFLKRDSYFRYLSDEDRIIRERVHCLPKAESVHVSVAAASIIARYVFLEEMEQLSRAVG-LLLPKGAGAIVDEAAARIIRARGEEMLETCAKLHFANTKKALAIAKRR

General information: TITO was launched using: RESULT: Template: 2D0B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1179 -104376 -88.53 -412.55 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -88.53 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.424

(partial model without unconserved sides chains): PDB file : Tito_2D0B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2D0B-query.scw PDB file : Tito_Scwrl_2D0B.pdb: