Modeling by threading (Tito software)
Unconserved sides chains calculation (Scwrl software)
Evaluation (QMean software)



Input alignment information:
Query sequenceMKIIVQKFGGTSVKDDKGRKLALGHIKEAISEGYKVVVVVSAMGRKGDPYATDSLLGLLYGDQSAISPREQDLLLSCGETISSVVFTSMLLDNGVKAAALTGAQAGFLTNDQHTNAKIIEMK-PERLFSVLANHDAVVVAGFQGATEKGDTTTIGRGGSDTSAAALGAAVDAEYIDIFTDVEGVMTADPRVVENAKPLPVVTYTEICNLAYQGAKVISPRAVEIAMQAKVPIRVRSTYS-NDKGTLVTSHHSSKVGSDVFERLITGIAHVKDVTQFKV--PAKIGQYNVQTEVFKAMANAGISVDFFNITPSE--IVYTVAGNKTETA----QRILMDMGYDPMVTRNCAK----------VSAVGAGIMGVPGVTSKIVSALSEK--EIPILQSADSHTTIWVLVHEADMVPAVNALHEVFELSK
2HMF Chain:B ((107-466))------------------------------------------------------LIGVAYLGE--LTPKSRDYILSFGERLSSPILSGAIRDLGEKSIALEGGEAGIITDNNFGSARVKRLEVKERLLPLLKEGIIPVVTGFIGTTEEGYITTLGRGGSDYSAALIGYGLDADIIEIWTDVSGVYTTDPRLVPTARRIPKLSYIEAMELAYFGAKVLHPRTIEPAMEKGIPILVKNTFEPESEGTLITNDM------EMSDSIVKAISTIKNVALINIFGAGMVGVSGTAARIFKALGEEEVNVILISQGSSETNISLVVSEEDVDKALKALKREFGDF-----LNNNLIRDVSVDKDVCVISVVGAGMRGAKGIAGKIFTAVSESGANIKMIAQGSSEVNISFVIDEKDLLNCVRKLHEKF----


General information:
TITO was launched using:
RESULT:

Template: 2HMF.pdb
Alignment : align.pir
Tito was launched with SMD and SCWRL
Tito text output
Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1645 -198673 -120.77 -596.62
target 2D structure prediction score : 0.65
Monomeric hydrophicity matching model chain B : 0.76

3D Compatibility (PKB) : -120.77
2D Compatibility (Sec. Struct. Predict.) : 0.65
1D Compatibility (Hydrophobicity) : 0.76
QMean score : 0.457

(partial model without unconserved sides chains):
PDB file : Tito_2HMF.pdb:





(Unconserved sides chains are recalculated) :
Sequence: align-2HMF-query.scw
PDB file : Tito_Scwrl_2HMF.pdb: