Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRLDKLLANSGYGSRKEVKAVVKAGAVMIDGKPAKDVKEHVDPDTQEVTVYGEPVDYREFIYLMMNKPQGVLSATEDSR-QQTVVDLLTPEEMRFEPFPAGRLDKDTEGFLLLTNDGQLAHRLLSPKKHVPKTYEVHLKSQISREDISDLETGVYIEG--------GYKTKPAKAEIK-----T-NDSGNTVIYLTITEGKYHQVKQMAKAVGNEVVYLKRLSMGRVSLDPALAPGEYRELTEEELHLLNEPQA 2OML Chain:A ((8-189)) --------------------------------------------------------NQPTRVILFNKPYDVLPQFTDEAGRKTLKEFIP----VQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPGKRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIR-RKSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLD-NLANGEWREVTD-----------

General information: TITO was launched using: RESULT: Template: 2OML.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 765 21918 28.65 132.03 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 28.65 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.574

(partial model without unconserved sides chains): PDB file : Tito_2OML.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2OML-query.scw PDB file : Tito_Scwrl_2OML.pdb: