Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKQCVAMLLAGGKGSRLSGLTKNMAKPAVSFG-GKYRIIDFTLSNCSNSGIDTVGILTQYQPLELNSYIGIGSAWDLDRYNG-GVTVLPPYAESSEVKWYKGTASSIYENLNYLNQYDPEYVLILSGDHIYKMDYGKMLDYHIEKKADVTISVIEVGWEEASRFGIMKANPDGTITHFDEKPKFP---------------------KSNLASMGIYIFNWPLLKQYLE-MDDQNPYSSHDFGKDIIPLLLEEKKKLSAYPFKGYWKDVGTVQSLWEANMDLLKEDSELKLFERKWKIYSVNPNQPPQFISSDAQVQDSLVNEGCVVYGNVSHSVLFQGVTVGKHTTVTSSVIMPDVTIGEHVVIENAIVPNGMVLPDGAVIRSEKDIEEVLLVSEEFVEKELI 1YP2 Chain:A ((18-298)) -SRSVLGIIL------RLYPLTKKRAKPAVPLGANY-RLIDIPVSNCLNSNISKIYVLTQFNSASLNRHLSRAYA-------EGFVEVLAAQQSPENPDWFQGTADAVRQYLWLFEEHTVLEYLILAGDHLYRMDYEKFIQAHRETDADITVAALPMDEKRATAFGLMKIDEEGRIIEFAEKPQGEQLQAMKVDTTILGLDDKRAKEMPFIASMGIYVISKDVMLNLLRDKFP----GANDFGSEVIPGATSLGMRVQAYLYDGYWEDIGTIEAFYNANLGITKKP----------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1YP2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1253 -17821 -14.22 -73.34 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -14.22 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.453

(partial model without unconserved sides chains): PDB file : Tito_1YP2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1YP2-query.scw PDB file : Tito_Scwrl_1YP2.pdb: