Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKNILLVGMLVLLLMFVSACSGTASKGSSSDSASEKTEMRTYKSPKG-NVNIPAHPKRIVTDF--YAGELLSVGANVVGSGSWS--FDNPFLKS--KLKNVKDVGD--PISVEKVMELQPDLIVVMNE------ENVDKLKKIAPTVVIPYNTAKNVEDTVSMFGDIAGAKDQAKSFMADFNKKAEAAKKKIAGVIDKDATFGIYENTDKGEFW-VFNDNGGRGGQAVYNALGLKAPEKIEQDVI---KKGEMKQLSQEVIPEYAADYMFITDYNPKGESKTLDKLENSSIWKNLDAVKHNRVFINDFDSFYPYDPISVSKQVDIITDMLIKRAEENKK 3R5T Chain:A ((3-291)) ------------------------------------NVWPRTFQNADGSITTIPSQPKRILSTAVTVTGTLLAIDAPVIASAATTQSTFFEQWRKLAELRQVKKLWPAGSVDLESVYVEQPDLIVVSMIGADSARDQIPLLQAIAPTILVDYSD-QTWQSLAQQLGLATGLEEQAERTIHNFEQWTKQVRDVLDL---PKGRANIVSYHGPGVVNAVAKAQ-SAHAQ-LLQSVGVVLEEPDPAWQAGSIVHRDFLRIHYEHLTQLQAETTFLITMTD----QQAQAFLHDPILKNLPSIQRKQVYGLGENSF-RIDLFSAREIINSLLRRFAGEQA----

General information: TITO was launched using: RESULT: Template: 3R5T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1455 56138 38.58 207.92 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 38.58 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.531

(partial model without unconserved sides chains): PDB file : Tito_3R5T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3R5T-query.scw PDB file : Tito_Scwrl_3R5T.pdb: