Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVRFLQISDLHFQFQNYD------TIVMRDKLLDFIEVLRRESDFDFLLLTGDIAHKGDVYNEDVKEYLNGIIK-----------SMG---------LSKNNVHLVPGNHDISRDMTRTLLIDSIMKSPNPSEMLDKLDQK-ATNILVEGQRKFFDFYEDFMGVKYPEEDLHFLYQSEKYNVLSINTCLLSDKKGEEGTLLTAQMKLYKAIRKMNKEKNKGKVLNIAIGHHTLGCIESSERESIKAHFDDYFIDLYLAGHVHDSSFNITANTNENPFLELVSGAIIKD-----EYATPEFISVDVNLDNGETEVTYYIWNTEYKYWSKNNQGGRRLQEGKLNYKINRLESLIEKEIEDNDDKIDEDEFKSFIIDFHEYRESYKTFTSNFDNQIGLDKKFYDMKSGETFKRKFDSYSEYFGVINHIMDSTSYVSADKKELIAETIVDKYLEFHNQYNNGDEIFVKIVNEIYLECHSVLPYSKALTKKYIKILTCWCIYECEIFNDNKRSVKND 4FBW Chain:A ((13-293)) -TIRILISSDPHVGYGEKDPVRGNDSFVSFNEILEIARE----RDVDMILLGGDIFHDNKPSRKALYQALRSLRLNCLGDKPCELELLSNINYLDPNINVAIPVFSIHGNHDD-----RYSAL--------------DILQVTGLVNYFGRV------P------NIVVSPILLQKGFTKLALYGISNVRDER---LYH--SFRENKVKFLRPDLYR-DE---WFNLLTVHQNHSAHTPTS-YLPESFI-QDFYDFVLWGHEHECLIDG-SYNPTQKFTVVQPGSTIATSLSPGETAPKHCGILNITGK------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4FBW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1163 15068 12.96 65.80 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 12.96 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.343

(partial model without unconserved sides chains): PDB file : Tito_4FBW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4FBW-query.scw PDB file : Tito_Scwrl_4FBW.pdb: