Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKQIISWITDYQNTGDEAVLRQVREVCWPIVEAVLQEKVMDDEQANNLREK--GIERFPFIISKYQADVQLPVETFLQNTYRFYFHQVMRESS 2DT6 Chain:A ((65-85)) ------------------------------------------PEFEARIRQNEINNPKFNFLN-------------------------------

General information: TITO was launched using: RESULT: Template: 2DT6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 16 -2433 -152.06 -128.05 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : -152.06 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.773

(partial model without unconserved sides chains): PDB file : Tito_2DT6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2DT6-query.scw PDB file : Tito_Scwrl_2DT6.pdb: