Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITLFQCLYLILFSFICYQGAAAFSHSTAASWLAAALGAAAAGLYIWNTKRVWKHCSSGLCAWIAVIQVMSVGVVLIGTDIMPVLCVIAIFAGCEGLRIGQSALQARLSDQIDKLTQAEQHANQMLIDVRSRNHDTMKHITAIKSA-Q----PKADTQAYIQNWADQYSQYDRFLK----------GENAYVAGVLYDFLEKARASNVSVSLHMHTPLSSLPFSPADQVSLVGNILENALDSAAEAREKAEIKLETSLRS----GLYVLTCENSTPGMDPKVLDTIYQSFGRSTKNGAHEGMGTYIIQK-LVKGAFGRLDFTYRHPIFRLEIKIPFQK 5EPV Chain:A ((17-210)) ----------------------------------------------------------------------------------------------------------------------------KALVSREIAHRFKNSMAMVQSIANQTLRNTYDPEQANRLFSERLRALSQAHDMLLKENWAGATIQQICATALAPFNS---TFANRIHMSGPH-LLVSDRVTVALSLAFYELATNAVKYGALSNEKGVINITWAIMEDKGEKKFHMRWAESRGPEVMQ---------------PARRGFGQRLLHSVLAEELKAKCDVEFAASGLLIDVLAPIT-

General information: TITO was launched using: RESULT: Template: 5EPV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 699 -86157 -123.26 -495.16 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : -123.26 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.270

(partial model without unconserved sides chains): PDB file : Tito_5EPV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EPV-query.scw PDB file : Tito_Scwrl_5EPV.pdb: