Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIHLSILDQAPVSKGESPVTTLQHSVELAQLSEQWGYKRYWFAEHHSTKG---LASTAPEIMIARIAAQTNTIRVGSGGVLLPQ-YSPFKVAETFRQLEALYPNRIDLGVGRSPGGTTKTRLALTD--GVKKSLTEFNRQLQDVSYFLTDS-LP-PDHPY---AGIKAAPLIGTAPELWVLGLGENSARRAAHQGIGYVFGHFINPER-GENAFRIYRESFRPSAHF-SNPSALFTIFVICAKTDEEAEELALSQDLWLLRVGKGLDSRVPSIEEAKAHPYTASDKKLIEENR-KRMVIGSPTTVKQQLLDLTGCYETNEIMVLCNVFDFEAKKESYERLAELFL 1RHC Chain:A ((2-325)) KTQIGYFASLEQ-------YRPMDALEQAIRAEKVGFDSVWVDDHFHPWYHDNAQSAQAWAWMGAALQATKKVFISTCITCPIMRYNPAIVAQTFATLRQMYPGRVGVAVGAGEAM--NEVPVTGEWPSVPVRQDMTVEAVKVMRMLWESDKPVTFKGDYFTLDKAFLYTKPDDEVPLYFSGMGPKGAKLAGMYGDHLMTVA-AAPSTLKNVTIPKFEEGAREAGKDPSKMEHAMLIWYSVDPDYDKAVEALRFWAGCLVPSMF-KYKVYD-PKEVQLH--A-NL-VHCDTIKENYMCATDAEEMIKEIERFK-EAGINHFCLGNSSP---DVNFGIDIF-KEVI

General information: TITO was launched using: RESULT: Template: 1RHC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1756 -165032 -93.98 -532.36 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -93.98 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.511

(partial model without unconserved sides chains): PDB file : Tito_1RHC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1RHC-query.scw PDB file : Tito_Scwrl_1RHC.pdb: