Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIAGYIMAAENQTLNQWTINQLGI----TRGDSILEVGFGPGYCMQQMLKREK-----DVHLHGIDVSEAMLKLAARRVKP---KGVRLIQGSIETFPLPASFYDKVISVNNYTIWNDQTKGIKQIYRALKPGGKAAITMQPREADAS----------P----E---------KT-KSFGRQMIADFKAAGFEDIDIQFKNIKPELSVCATAKKPAT 5EGP Chain:A ((58-241)) --------------AQTLVDYSGVANTSQKPLVVLDNACGIGAVSSVLNHTLQDEAKKTWKLTCGDLSEGMLETTKRRLQDEGWVNAETKIVNALDTGLPDGHYTHVFVAFGFQSFPDANAALKECFRILASGGILASSTWQNFNWIPIMKAAIETIPGNLPFPTQKEFITLHNAGWDSESYIQSELEKLGFRDVKVI-------------------

General information: TITO was launched using: RESULT: Template: 5EGP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 731 -79036 -108.12 -534.03 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -108.12 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.506

(partial model without unconserved sides chains): PDB file : Tito_5EGP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EGP-query.scw PDB file : Tito_Scwrl_5EGP.pdb: