Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTASQQQIQLARRPQGIPVHEDFRFET----IPVPEPKQGEVLVKTLYVSVDPYMRGRM----QDTKSYVEPFALDKALSGGVIAEVVSDGN-HLKKGDIVIGNLSWQEFSAVS--ESALRKIDTSLAPASAYLGILGMTGLTAYFGLLDIGRPKEGETVVVSGAAGAVGSTVGQIAKIKGARVVGIAGSDEKIDYLKQELQFDEAINYKTADDIQKALQNACPDGVDVYFDNVGGPISDAVMNLLNEFARIPVCGAISSYNAESEADDMGPRVQSKLIKTKSLMQGFIVSDYSDRFSEGAKQLAEWLKAGKLHYEETITEGFENIPDAFLGLFKGENKGKQLIKVSDPS 2J3I Chain:B ((1-343)) MTATNKQVILKDYVSGFPTESDFDFTTTTVELRVPE-GTNSVLVKNLYLSCDPYMRIRMGKPDPSTAALAQAYTPGQPIQGYGVSRIIESGHPDYKKGDLLWGIVAWEEYSVITPMTHAHFKIQHTDVPLSYYTGLLGMPGMTAYAGFYEVCSPKEGETVYVSAASGAVGQLVGQLAKMMGCYVVGSAGSKEKVDLLKTKFGFDDAFNYKEESDLTAALKRCFPNGIDIYFENVGGKMLDAVLVNMNMHGRIAVCGMISQYNLENQE---GVHNLSNIIYKRNRIQGFVVSDFYDKYSKFLEFVLPHIREGKITYVEDVADGLEKAPEALVGLFHGKNVGKQVVVVA---

General information: TITO was launched using: RESULT: Template: 2J3I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1943 -204946 -105.48 -617.31 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : -105.48 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.500

(partial model without unconserved sides chains): PDB file : Tito_2J3I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2J3I-query.scw PDB file : Tito_Scwrl_2J3I.pdb: