Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MDYLSLSLTMIFVLIAL-FLSKSFKAGVEKDMIIATIRAAVQLLIIGYVLSLIF-RGDHP-VFILLMVLLMLAVAAQNVIKRKKNTIGSFWRVFAALAIVEIVTQG---ILLSLHIIPLTAR---------YVIPISGMVIGNSMVL---SSLFLNRLNSEVGVRKEEIQLILS---LGGTPKQSIQRILTSAMKMSMIPTLESQKTLGLVQLPGM---------MTGQILAGADPIQAVRFQL-LIVFT------------TMASAALTCVILSVLTYPSLFTVHQQLKQNE 3GN6 Chain:A ((22-321)) FNPWTDAALDTIVNQALTLYAEMRVVPAHHDAFLAA--IDTVSAKLRVLPGFLSLALKQMSGDSTMVKNYPETYKGV-LATAYLDGVAAGTQPYFYNLFVRFADGRAARAAGFEALFETHIHPLLHAMADGPELLAYRAVLQSVVAGDRHAIYRGAEEIRSFLRRPVELPERETVTVENHVMVPEDKHAAWEPQVAILLQVAQD-TFEPQDEPSGVGLPGARDNRYYRKALSTEILRNAHADGGLRAYIMHGVWESVWDHENSHLDPRFLAAAGPVGAAAVVGPVEPFYLTRRLVVAD

General information: TITO was launched using: RESULT: Template: 3GN6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1110 -143300 -129.10 -582.52 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -129.10 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.170

(partial model without unconserved sides chains): PDB file : Tito_3GN6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GN6-query.scw PDB file : Tito_Scwrl_3GN6.pdb: