Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MALFTAKVTARGGRAGHITSDDGVLDFDIVMPNAKKEGQTGTNPEQLFAAGYAACFGGALEHVAKEQNIEIDS-EIEGQVSLMKDESDGGFKIGVTLVVNTKDLDREKAQELVNAAHEFCPYSKATRGNVDVKLELK 3EER Chain:A ((11-143)) -TIYQTSATASAGRNGVVSTEDKLLELNLSYPKEMGGSGTATNPEQLFAVGYAACFSNAILHVAREAKVALKEAPVTATVGIGPNGQ-GGFALSVALAAHI-ALEDEQARQLVTVAHQVCPYSNAVRGNIDVQVSV-

General information: TITO was launched using: RESULT: Template: 3EER.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 492 -85192 -173.15 -645.39 target 2D structure prediction score : 0.79 Monomeric hydrophicity matching model chain A : 0.85 3D Compatibility (PKB) : -173.15 2D Compatibility (Sec. Struct. Predict.) : 0.79 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.567

(partial model without unconserved sides chains): PDB file : Tito_3EER.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EER-query.scw PDB file : Tito_Scwrl_3EER.pdb: