Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTHSFAVPRSVEWKETAITILNQQKLPDETEYLELTTKEDVFDAIVTLKVRGAPAIGITAAFGLALAAKDIE----TDNVTEFRRRLEDIKQYLNSSRPTAINLSWALERLSHSVENAI---SVNEAKTNLVHEAIQIQVEDEETCRLIGQNALQLF--------KKGDRIMTICNAGSIATSRYGTALAPFYLAKQKDLGLHIYACETRPVLQGSRLTAWELMQGGIDVTLITDSMAAHTMKEKQISAVIVGADRIAKNGDTANKIGTYGLAILANAFDIPFFVAAPLSTFDTKVKCGADIPIEERDPEEVRQISGV----RTAPSNVPVFNPAFDITPHDLIS-GIITEKGIMTGNYEEEIEQLFKGEKVH 2A0U Chain:A ((15-370)) ----HATLESIKYTPGSLRLLDQRKLPLETVFDDVLTVEDIWSAIKEMRVRGAPAIAVSAALGIAVATQRKAANGELKSGREVQTFLLTSCDFVMTSRPTAVNLFNCLRDLKAQVDKLDPTKAAAEVAQAFVELAEAVYTNDVAFNEGIMRHGAAHILAAAKAEGRDKVSILTICNTGALATSRYGTALGVVRQLFYDGKLERVYACETRPWNQGARLTVYECVQEDIPCTLICDGAASSLMLNRKIDAVVVGADRICQNGDTANKIGTYNLAVSAKFHGVKLYVAAPTTTLDVKTASGNHVEIEEREPTEITTNLVTKQRVVADGPHLSIWNPVFDITPSELITGGIITEKGVQAPAAS-------------

General information: TITO was launched using: RESULT: Template: 2A0U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2030 -184166 -90.72 -548.11 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -90.72 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.545

(partial model without unconserved sides chains): PDB file : Tito_2A0U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2A0U-query.scw PDB file : Tito_Scwrl_2A0U.pdb: