Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGRQVKCPYCETKLDKDSAIPYKK--RYYHEKCFNTWKQESDHRKELIQYICNLYGLTSPTGMMLKQIKEFQEEYGYKLKGIELALRYFYETLDNQPREGDGIGIVPFVYDEAKRHYIRQKAIRKSAEDPKNHKREEITLVIKKGMRKKRGLVDISML 5D1M Chain:B ((4-130)) NIPHGQCVICLYGFQEKEAFTKTPCYHYFHCHCLARYIQHMEQELKAQGGV---------------QCAVCREPLVYDLASLKAAPEPQQPMELYQP-------------------------------SAESLRQQEERKRLYQRQQERGGIIDLE--

General information: TITO was launched using: RESULT: Template: 5D1M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 296 -13916 -47.01 -128.85 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain B : 0.62 3D Compatibility (PKB) : -47.01 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.272

(partial model without unconserved sides chains): PDB file : Tito_5D1M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5D1M-query.scw PDB file : Tito_Scwrl_5D1M.pdb: