Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLNQKA--LNSYVYTLAFSSLSFGLIFGLYLFVYSGFMAIALVTIAIIAFYALITYLVFAAPLQVWLRRRRRKFSL---INFLIYIAVAFSAVFLFWFVDY-PPNALTMFRSFEYYIMSIVAAFIYWFWDSIFLRN 1VK5 Chain:A ((36-156)) GSLLRRAEMYQDYMKQVPIPTNRGSLI---------PFTSWVGLSISMKQLYGQPLHYLTNVLLQRWDQSRFGTDSEEQRLDSIIHPTKAEATIWLVEEIHRLTPSHLHMALLWRSDPM--YHSFI----DPIFPE-

General information: TITO was launched using: RESULT: Template: 1VK5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 390 -58312 -149.52 -507.06 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -149.52 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.171

(partial model without unconserved sides chains): PDB file : Tito_1VK5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VK5-query.scw PDB file : Tito_Scwrl_1VK5.pdb: