Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMTGTLGTLVPIILMFAVLYFLLIRPQQKQQKAVRQMQEELKKGDSVVTIG----GLHGTVDSIDES--KVVIKTG--DN-TRLTFDRRAIREVSAAE 2LQ8 Chain:A ((120-174)) -------------------------------------ELGFKVGDMVKIISGPFEDFAGVIKEIDPERQELKVNVTIFGRETPVVLHVSEVE------

General information: TITO was launched using: RESULT: Template: 2LQ8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 119 9218 77.46 200.38 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : 77.46 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.501

(partial model without unconserved sides chains): PDB file : Tito_2LQ8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2LQ8-query.scw PDB file : Tito_Scwrl_2LQ8.pdb: