Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------MKKRKNKKNSKAAEKALKVTINGKEETVYEQETPETEANKSMTFSN-----WEEKRQAEQEVAASQEHPDEDEFNWDSEE------DKVFKED---------------PKVVPPFQKKKTKLYAKGKTGAAKP--VKRVAATIAFAAVIGTGLGLFALNISGN-----KEASAPASLEDSLGSQTAKAGDTSADKQTSGAEKQAAQTE------------GTYKTYAVQAGKFSNEKGAE--TLTEQLTEKGYSAVSLSKDDGYTYVIA---------GLASEKEVSQQLGQVLIDS-------DFEAWGGKELSLSIESDMT-DSFKETAELAAKAILDE---DITKASVEK------------IEKSLGETKASETGEKKAILQALKELEDPSAEAGWKAQQ----ELLAVVK---------------------------------------------- 4EWV Chain:A ((14-581)) NETFEKQLKDLTSNVKSIQDNLLEEIITPDKELFKKNVSYEDIKPYLDRVVNGESQKMMPWNNKYLDNLTFIYDLRMQVITKHVKGVEMMFLFTKQEPARVATSSYFKSDYFKNRPSNWYYSYTSPDEVILCPNNTESLYCHLLCGLVQRGSIFASVMVRAIEVLKNSWEELCSNIRSGHLSNWVTDLGCQNSVSLVLGGPRPELADTIEEICNQNSWKGIVKRLWPNTKYIETVVTGSMGQYVPMLNYYCNDLPLVSTTYGSSETTFGINLDPLCKPEDVSYTFMPNMSYFEFIGCTYAGLYRMRVGDIVLVTGFYNNAPQFKFVRRENVVLSIDSDKTNEEDLFKAVSQAKLVLESSGLDLKDFTSYADTSTFPGHYVVYLEVDTKQFELDEEALSTCCLVMEESLDNVYKRCRFKDGSIGPLEIRVVRQGTFDSLMDFFISQGASTGQYKTPRCIKSGKALQVLETCVVAKFFSI

General information: TITO was launched using: RESULT: Template: 4EWV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1412 119973 84.97 361.36 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 84.97 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.184

(partial model without unconserved sides chains): PDB file : Tito_4EWV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EWV-query.scw PDB file : Tito_Scwrl_4EWV.pdb: